Tracking polyhydroxyalkanoate biosynthesis in thermophilic microorganisms
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资源简介：
"Tracking PHA biosynthesis in thermophiles: supplementary dataset"
This dataset accompanies the publication "Tracking PHA biosynthesis in thermophiles" and contains comprehensive data used in the analysis of polyhydroxyalkanoate (PHA) synthase (PhaC) sequences across archaeal and bacterial thermophiles. The provided data includes sequence alignments, classifications, and detailed lists of putative and verified PhaC sequences identified through bioinformatic analyses.
Contents:
Supplementary File S1: A categorized list of archaeal and bacterial genera/classes based on optimal growth temperature.
Supplementary Table S1: Detailed inventory of microorganisms from which PhaC sequences were utilized in bioinformatic analyses.
Supplementary Figure S1: Multiple sequence alignment visualization of 16 well-characterized or automatically annotated reference PhaC sequences.
Supplementary Figure S2: Matrix illustrating pairwise percent identities among aligned PhaC protein sequences.
Supplementary File S2: Identification and annotation of putative PhaC sequences from bacterial moderate thermophiles, highlighting discrepancies in lipase-like box motif alignments.
Supplementary File S3 (Zenodo): Complete multiple sequence alignment of 366 putative bacterial moderate thermophilic PhaC sequences, including the 16 reference sequences.
Supplementary Table S2: Curated and filtered dataset of bacterial moderate thermophilic PhaC sequences selected for further analysis.
Supplementary File S4: Identification and annotation of putative PhaC sequences from archaeal moderate thermophiles, including details on lipase-like box motif alignments.
Supplementary File S5 (Zenodo): Complete multiple sequence alignment of 256 putative archaeal moderate thermophilic PhaC sequences alongside the 16 reference sequences.
Supplementary Table S3: Curated and filtered dataset of archaeal moderate thermophilic PhaC sequences selected for further analysis.
Supplementary File S6: Identification and annotation of putative PhaC sequences from bacterial extreme thermophiles, highlighting discrepancies in lipase-like box motif alignments.
Supplementary File S7 (Zenodo): Complete multiple sequence alignment of 164 putative bacterial extreme thermophilic PhaC sequences, including the 16 reference sequences.
Supplementary File S8: Identification and annotation of putative PhaC sequences from archaeal extreme thermophiles, highlighting discrepancies in lipase-like box motif alignments.
Supplementary File S9 (Zenodo): Complete multiple sequence alignment of 26 putative archaeal extreme thermophilic PhaC sequences alongside the 16 reference sequences.
Supplementary Table S4: Curated and filtered dataset of archaeal extreme thermophilic PhaC sequences selected for further analysis.
Supplementary Figures S3-S5: Analytical results of PHA characterization, including gas chromatography-mass spectrometry (GC-MS), differential scanning calorimetry (DSC), and proton nuclear magnetic resonance (¹H-NMR).
DOI: 10.5281/zenodo.15221919
嗜热菌PHA生物合成追踪：补充数据集
本数据集配套于论文《嗜热菌聚羟基脂肪酸酯（Polyhydroxyalkanoate, PHA）生物合成追踪》，收录了用于跨古菌与细菌嗜热菌株的PHA合酶（PHA synthase, PhaC）序列分析的全套数据。所提供的数据涵盖序列比对、分类结果，以及通过生物信息学分析鉴定得到的推定与已验证PhaC序列的详细列表。
数据集内容：
Supplementary File S1: 基于最适生长温度分类的古菌与细菌属/纲列表。
Supplementary Table S1: 本研究生物信息学分析中所用PhaC序列来源微生物的详细清单。
Supplementary Figure S1: 16条经过充分表征或自动注释的参考PhaC序列的多序列比对可视化结果。
Supplementary Figure S2: 比对后的PhaC蛋白序列间两两百分比一致性的矩阵可视化结果。
Supplementary File S2: 细菌中度嗜热菌推定PhaC序列的鉴定与注释，重点标注了脂酶样框基序比对中的不一致之处。
Supplementary File S3 (Zenodo): 366条细菌中度嗜热菌推定PhaC序列的完整多序列比对结果，包含上述16条参考序列。
Supplementary Table S2: 经筛选整理的细菌中度嗜热菌PhaC序列数据集，用于后续分析。
Supplementary File S4: 古菌中度嗜热菌推定PhaC序列的鉴定与注释，包含脂酶样框基序比对的详细信息。
Supplementary File S5 (Zenodo): 256条古菌中度嗜热菌推定PhaC序列与上述16条参考序列的完整多序列比对结果。
Supplementary Table S3: 经筛选整理的古菌中度嗜热菌PhaC序列数据集，用于后续分析。
Supplementary File S6: 细菌极端嗜热菌推定PhaC序列的鉴定与注释，重点标注了脂酶样框基序比对中的不一致之处。
Supplementary File S7 (Zenodo): 164条细菌极端嗜热菌推定PhaC序列的完整多序列比对结果，包含上述16条参考序列。
Supplementary File S8: 古菌极端嗜热菌推定PhaC序列的鉴定与注释，重点标注了脂酶样框基序比对中的不一致之处。
Supplementary File S9 (Zenodo): 26条古菌极端嗜热菌推定PhaC序列与上述16条参考序列的完整多序列比对结果。
Supplementary Table S4: 经筛选整理的古菌极端嗜热菌PhaC序列数据集，用于后续分析。
Supplementary Figures S3-S5: PHA表征分析结果，涵盖气相色谱-质谱联用（GC-MS）、差示扫描量热法（DSC）以及质子核磁共振（¹H-NMR）检测数据。
DOI: 10.5281/zenodo.15221919
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Zenodo创建时间：
2025-12-10
