Genomes of six pomegranate accessions, including genome fasta, cds fasta, pep fasta, and gff

Pomegranate (<i>Punica granatum</i> L.) is one of the most ancient edible fruit tree species. Here we reported a new chromosome-level genome assembly and annotation of sour pomegranate. We assembled the genome with a size of 331.47 Mb and used BUSCO to estimate the completeness of the assembly as 98.8%. More than 97.40% of sequences in the final assembly were anchored to 8 pseudochromosomes, higher than the corresponding percentages for the existing reference genomes ‘Tunisia’ (92.62%). Using a combination of de novo prediction, protein homology and RNA-seq annotation, 29,326 protein-coding genes were predicted. We re-annotated the protein-coding genes of five other published pomegranate genomes using the same annotation method. We constructed the pan-genome of pomegranate using protein-coding genes, integrating data from our newly assembled genome and five other published genomes. The pan-genome was composed of 28,314 gene families, of which 68.96% were core genes, 30.00% were dispensable genes, and 1.04% were private genes. The chromosome-level reference genome of sour pomegranate would be valuable resource for research and molecular breeding of pomegranate.

石榴（<i>Punica granatum</i> L.）是最古老的可食用果树物种之一。本研究报道了一份全新的酸石榴染色体级基因组组装与注释结果。本次组装的基因组大小为331.47 Mb，通过BUSCO评估其组装完整性达98.8%。最终组装序列中超过97.40%被锚定至8条假染色体上，这一比例高于现有参考基因组“突尼斯”（Tunisia）的92.62%。我们结合从头预测、蛋白质同源性比对及RNA-seq注释三种方法，共预测得到29326个蛋白质编码基因。我们采用相同的注释方法，对另外5个已发表的石榴基因组的蛋白质编码基因进行了重新注释。我们以蛋白质编码基因为基础，整合本次新组装的基因组与其余5个已发表的石榴基因组数据，构建了石榴泛基因组。该泛基因组共包含28314个基因家族，其中68.96%为核心基因，30.00%为附属基因，1.04%为特有基因。这份酸石榴染色体级参考基因组将为石榴的相关研究与分子育种提供宝贵的遗传资源。