Integrated multi-cohort single-cell atlas of osteoarthritic chondrocyte remodeling

This dataset contains a processed multi-cohort single-cell RNA-sequencing atlas of osteoarthritic chondrocyte remodeling generated through integration of publicly available osteoarthritis cartilage datasets. The release includes a frozen harmonized single-cell atlas used for manuscript analyses, curated cell-state annotations, dataset and condition summaries, cross-cohort robustness analyses, and transcriptome-informed therapeutic vulnerability prioritization outputs. The principal dataset included in this release is: publication_clean_atlas_FINAL_FROZEN.h5ad This harmonized atlas was used for downstream analyses including: • disease-associated chondrocyte state annotation• branch-specific remodeling trajectory reconstruction• signaling program characterization• transcriptome-informed therapeutic vulnerability prioritization• cross-cohort reproducibility analyses Raw sequencing data are not redistributed in this release and remain available through their original Gene Expression Omnibus (GEO) repositories referenced in the associated manuscript. Included supporting materials: • cell-state annotation tables• dataset and condition summaries• cross-cohort robustness tables• therapeutic vulnerability landscape tables• supporting documentation for reproducibility This release corresponds to the processed atlas version used in the associated manuscript.本数据集包含经处理的骨关节炎软骨细胞重塑多队列单细胞RNA测序（single-cell RNA-sequencing）图谱，该图谱通过整合公开可用的骨关节炎软骨数据集构建而来。 本次发布包含用于论文分析的固定版整合单细胞图谱、经人工整理注释的细胞状态注释文件、数据集与实验条件汇总资料、多队列稳健性分析结果，以及基于转录组信息的治疗靶点脆弱性优先级排序输出成果。 本次发布的核心数据集为：publication_clean_atlas_FINAL_FROZEN.h5ad 该整合图谱被用于以下下游分析： • 疾病相关软骨细胞状态注释 • 分支特异性重塑轨迹重构 • 信号通路特征解析 • 基于转录组信息的治疗靶点脆弱性优先级排序 • 多队列可重复性分析 本次发布未提供原始测序数据，相关原始数据可通过关联论文中引用的基因表达综合数据库（Gene Expression Omnibus，GEO）仓库获取。 配套支持材料包括： • 细胞状态注释表格 • 数据集与实验条件汇总资料 • 多队列稳健性分析表格 • 治疗靶点脆弱性全景分析表格 • 可重复性研究支持文档 本次发布对应关联论文中使用的处理后图谱版本。