Transcriptome sequencing of the hepatic samples in antibiotic-treated mice under normal light-dark cycle and short-day

Intestinal microbiota dysbiosis is related to many metabolic diseases in human health. Meanwhile, as an irregular environmental light-dark cycle, short-day (SD) may induce host circadian rhythms disturbances and worsen the risks of gut dysbiosis. Herein, we investigated how LD cycles regulate intestinal metabolism upon the destruction of gut microbes with antibiotic treatments. The transcriptome data indicated that SD have some negative effects on hepatic metabolism, endocrine, digestive, and diseases processes compared with normal light-dark cycle (NLD).The SD induced epithelial and hepatic purine metabolism pathway imbalance in ABX mice, the gut microbes, and their metabolites, all of which could contribute to host metabolism and digestion, endocrine system disorders, and may even cause diseases in the host. Overall design: The antibiotic-treated mice were managed under alternating photoperiods of normal light-dark cycle (NLD, 12-hour light /12-hour dark, n=3) and short day (SD, 8-hour light / 16-hour dark, n=3). Mice were then anesthetizedwith ether andeuthanizedby spinal dislocation, the liver samples were collected for transcriptome sequencing.