Reproduction, growth and oxidative stress in the earthworm Eisenia andrei exposed to conventional and biodegradable mulching film microplastics

Dataset on Earthworm (Eisenia andrei) exposed to of low-density polyethylene microplastics (PE-MP) and polybutylene adipate terephthalate biodegradable microplastics (PBAT-BD-MP) originating from mulching films. The study utilized seven concentrations (0, 0.005, 0.05, 0.1, 0.5, 1, and 5% w/w) in five replicates. Survival, growth, and oxidative stress responses of the earthworms were analysed after four weeks of exposure. Measured biomarker responses were superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase, glutathione, and lipid peroxidation. Additionally, the Integrated Biomarker Response Index (IBR) was conducted to assess the overall oxidative stress status of the earthworms. The number of biomass of the juveniles were recorded after four additional weeks. The total length of the experiment was eight weeks. Soil water holding capacity (WHC) was analysed in the beginning of the experiment and soil pH in the beginning and end of the experiment. The study was conducted at the Finnish Environment Institute (Syke) Under project PAPILLONS (EU Horizon).本数据集聚焦于暴露于源自地膜的低密度聚乙烯微塑料（low-density polyethylene microplastics，PE-MP）与聚己二酸对苯二甲酸丁二酯可降解微塑料（polybutylene adipate terephthalate biodegradable microplastics，PBAT-BD-MP）的赤子爱胜蚓（Eisenia andrei）。本研究设置7个浓度梯度（0、0.005、0.05、0.1、0.5、1及5% 重量/重量比），每组设置5个重复样本。暴露4周后，分析蚯蚓的存活率、生长情况及氧化应激响应。本次检测的生物标志物包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽S-转移酶、谷胱甘肽以及脂质过氧化产物。此外，通过整合生物标志物响应指数（Integrated Biomarker Response Index，IBR）评估蚯蚓的整体氧化应激状态。再经过4周饲养后，记录幼蚓的数量与生物量。整个实验周期总计8周。分别于实验起始时测定土壤持水量（water holding capacity，WHC），并于实验起始与结束时测定土壤pH值。本研究由芬兰环境研究所（Finnish Environment Institute，Syke）在欧盟地平线计划（EU Horizon）旗下的PAPILLONS项目框架下开展。