In vivo cells from liver and brain from mice treated with control and keto diet collected in different ages - 12 and 26 months

Beta-hydroxybutyrate (BHB) is a ketone body synthesized during fasting or strenuous exercise. Our previous study demonstrated that a cyclic ketogenic diet (KD), which induces BHB levels similar to fasting every other week, reduces midlife mortality and improves memory in aging mice. First, we analyzed hepatic gene expression in an aging KD-treated mouse cohort using bulk RNA-seq. In addition to the downregulation of TOR pathway activity, cyclic KD reduces inflammatory gene expression in the liver. We examined the brain gene expression of 12-month-old male mice fed with one-week and one-year cyclic KD. While a one-week KD increases inflammatory signaling, a one-year cyclic KD reduces neuroinflammation induced by aging. 16 samples of liver from 26 months old mice, 8 exposed to control diet and 8 exposed to keto diet. 28 samples from mouse brain. 12 mice 12 months old - 5 control diet and 7 keto diet. 16 samples from mice 26 months old, 8 control diet e 8 treated with keto diet.