CIL:46205, Homo sapiens. In Cell Image Library

Plate 24319 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

孔板编号24319——表型谱分析（phenotypic profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行整合总结，使谱间的相似性能够反映样本间的相似性。该图像集为基于图像的表型谱分析方法（image-based profiling methods）在区分小分子化合物作用效果的能力测试中提供了基准数据集。

图像数据集说明
本图像集的成像对象为经1600种已知生物活性化合物分别处理的U2OS人骨肉瘤细胞，使用6种标记物对7种细胞器进行染色，该实验采用细胞绘画检测法（cell-painting assay）。

本次预实验共包含20块384孔板。每块孔板设有384个样品孔，每个样品孔采集9个成像视野，总计69120个成像视野。每个视野通过5个成像通道（检测波长）完成成像，每个通道的图像均以独立的灰度图像文件存储，因此该数据集共包含345600个16位TIFF格式的图像文件。