Peak viremia and presence of neutralizing anti-WNV antibodies (nAb) in sera of HOFIs before infection and on day of death.
收藏资源简介:
All HOFI sera were tested for nAb at a 1:20 dilution before infection and at the time of death with the end of the project at 14 dpi. Neutralization of >90% of 50–90 PFU was considered positive using a plaque reduction neutralization assay (PRNT90). Peak viremia titers determined by plaque assay titration are shown as log10 PFU/mL and occurred on 3 dpi or 4 dpi.Peak viremia and presence of neutralizing anti-WNV antibodies (nAb) in sera of HOFIs before infection and on day of death.
所有HOFI来源的血清均在感染前以1:20的稀释度进行中和抗体(nAb,neutralizing antibody)检测,检测同时覆盖动物死亡时刻以及本项目结题时的感染后14天(14 dpi)。采用空斑减少中和试验(PRNT90,plaque reduction neutralization assay)判定结果时,若对50~90个蚀斑形成单位(PFU,plaque forming unit)的中和抑制率超过90%,则判定为阳性。通过空斑滴定法测定的病毒血症峰值滴度以log10 PFU/mL为单位展示,其出现时间为感染后3天(3 dpi)或感染后4天(4 dpi)。本数据集收录了HOFI个体感染前及死亡时刻血清中的病毒血症峰值数据与抗西尼罗病毒(WNV,West Nile Virus)中和抗体的存在情况。



