Expression profile of sweat gland label retaining cells (LRCs). Mus musculus

We have used the slow cycling property, found in hair follicle stem cells, to look for LRCs in sweat glands as putative stem cells. Gene expression profiling allowed us to determine the common and unique genes expressed in SG LRCs and non-LRCs. This allowed us to probe for the signaling pathways active in this skin appendage as well as determine potential molecular markers of SG LRCs. Overall design: Sweat gland label retaining cells (LRCs) and surrounding basal layer cells were isolated from K5TetOffTreH2BGFP mice after a 4 week chase with doxycycline and compared to the basal layer of the sole's epidermis in 2 independent experiments.我们利用在毛囊干细胞（hair follicle stem cells）中发现的慢循环特性，在汗腺中搜寻作为推定干细胞的滞留标记细胞（label retaining cells, LRCs）。通过基因表达谱分析，我们得以鉴定汗腺滞留标记细胞（SG LRCs）与非滞留标记细胞（non-LRCs）中表达的共有基因与特有基因。借此我们既可探究该皮肤附属器内活跃的信号通路，亦可确定汗腺滞留标记细胞（SG LRCs）的潜在分子标志物。 实验设计概述：经多西环素处理并进行4周标记追踪后，从K5TetOffTreH2BGFP小鼠体内分离出汗腺滞留标记细胞（LRCs）及其周围基底层细胞，并在2组独立实验中与足底表皮基底层进行对照比较。