Genome-wide effect of constitutive IFN-signaling. Genome-wide effect of constitutive IFN-signaling

Using wild type mice and mice deficient for either IFNb1 or IFN-receptor, we analysed the effect of the constitutive IFN on genome-wide expression Overall design: To look for the role of constitutive interferon, we compared levels of expression of Interferon-stimulated genes (ISGs) in alveolar macrophages or liver in three mouse lines that is, C57BL6, IFNb1-KO, and IFN-receptor KO mice. Specifically, we isolated RNA from rested alveolar macrophages or liver and made library using Illumina TruSeq kit for the library construction. One replica of each sample is provided.本研究以野生型小鼠（wild type mice）、IFNβ1缺陷型小鼠以及干扰素受体（IFN-receptor）缺陷型小鼠为实验对象，分析了组成型干扰素对全基因组表达谱的影响。实验整体设计：为探究组成型干扰素的生物学作用，我们在C57BL6、IFNβ1敲除（IFNb1-KO）、干扰素受体敲除小鼠这3个小鼠品系的肺泡巨噬细胞或肝脏组织中，对比了干扰素刺激基因（Interferon-stimulated genes，ISGs）的表达水平。具体实验流程为：从静置培养的肺泡巨噬细胞或肝脏组织中提取总RNA，采用Illumina TruSeq建库试剂盒（Illumina TruSeq kit）完成文库构建。本数据集包含每个样本的1个重复样本。