Telomerase is essential for cardiac differentiation and sustained metabolism of human cardiomyocytes

The scRNA seq was performed on day 12 of hiPSC-cardiomyocyte differentiation protocol with 3 different conditions of telomere length at the hiPSC stage, namely- long telomeres, intermediate telomeres and short telomeres. The analysis showed expected clusters of the mesodermal lineage but a shift of clusters was observed depending upon the telomere legth status of the parent CRISPRi TERT hiPSCs used for differentiation The scRNA seq was performed on day 12 of hiPSC-cardiomyocyte differentiation protocol with 3 different conditions of telomere length at the hiPSC stage, namely- long telomeres, intermediate telomeres and short telomeres. This was performed in a doxycycline inducible CRISPRi TERT hiPSC line.

本研究针对诱导多能干细胞（human induced pluripotent stem cells, hiPSC）向心肌细胞分化方案的第12天样本开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq），样本基于hiPSC阶段的端粒长度分为3组，即长端粒组、中端粒组与短端粒组。分析结果可检测到预期的中胚层谱系细胞簇，但细胞簇的分布偏移情况与用于分化的亲本CRISPR干扰端粒酶逆转录酶（CRISPRi TERT）hiPSCs的端粒长度状态密切相关。本次实验同样采用scRNA-seq技术，针对诱导多能干细胞向心肌细胞分化方案的第12天样本开展检测，样本按hiPSC阶段的端粒长度分为长端粒、中端粒及短端粒3组。该实验体系依托多西环素诱导型CRISPR干扰端粒酶逆转录酶（CRISPRi TERT）hiPSC细胞系构建。