Glucocorticoids delay RAF-induced senescence promoted by EGR1

Analysis of the transcriptional response to treatment with glucocorticoids in human fibroblasts entering oncogene-induced senescence. BJ-hTert-B-RAF-V600E human cells treated for 24-120 hours with doxycycline to induce B-Raf-V600E expression were treated with clobetasol or compound A for 2 hours before harvest or during the entire time with doxycycline. At least three replicates per sample. Differential expression with clobetasol assessed against control with DMSO (vehicle).进入癌基因诱导性衰老的人成纤维细胞经糖皮质激素（glucocorticoids）处理后的转录应答分析。本研究采用经多西环素（doxycycline）处理24~120小时以诱导B-Raf-V600E表达的BJ-hTert-B-RAF-V600E人细胞，分别在收获前2小时或全程联合多西环素处理时，施加氯倍他索（clobetasol）或化合物A进行干预。每份样本设置至少3次生物学重复。以二甲基亚砜（DMSO，溶剂对照）为参照，评估氯倍他索处理组的基因差异表达情况。