shRNA screen of factors involved in Aire-sensitive gene expression on their 3'UTR lengthening. shRNA screen of factors involved in Aire-sensitive gene expression on their 3'UTR lengthening

CLP1 controls the expression of Aire-sensitive genes with proximal pAs and their shortening in HEK293 cells Overall design: Specific knockdown of CLP1, HNRNPL, DDX5, DDX17, PARP1, PRKDC, SUPT16H, PABPC1, CPSF6 and the control LacZ gene in HEK293 cells

CLP1可调控HEK293细胞中携带近端多聚腺苷酸化位点（proximal pAs）的Aire敏感基因（Aire-sensitive genes）的表达，以及此类基因的转录本3'端缩短现象。实验整体设计：在HEK293细胞中对CLP1、HNRNPL、DDX5、DDX17、PARP1、PRKDC、SUPT16H、PABPC1、CPSF6及对照基因LacZ进行特异性敲低。