Gene expression signature of the T-ALL cell line LOUCY treated with 250nM (+)-JQ1 for 4h

We analyzed the transcriptional consequences of the BET bromodomain inhibitor JQ1 in the T-ALL cell line LOUCY by microarray analysis. Short-term exposure to a low dose of JQ1 (4h, 250nM) provided insights in the genes whose expression was immediately affected by BET bromodomain inhibition. Significantly downregulated genes upon short-term drug treatment included stem-cell associated genes and putative oncogenes such as BAALC, WT1, MN1, MEF2C, LMO1 and LMO2. Genes associated with the 500 highest ranked enhancer regions in LOUCY, were significantly enriched in genes downregulated after JQ1 treatment. LOUCY cells were treated with 250nM (+)-JQ1 or DMSO for 4h. Three biological replicates of this treatment were performed.

本研究通过微阵列分析（microarray analysis），探究了BET溴结构域抑制剂（BET bromodomain inhibitor）JQ1对T细胞急性淋巴细胞白血病（T-cell acute lymphoblastic leukemia, T-ALL）细胞系LOUCY的转录层面影响。以250nM低剂量JQ1短期处理细胞4小时，可为解析即刻受BET溴结构域抑制调控的基因提供思路。短期药物处理后显著下调的基因包括干细胞相关基因及推定致癌基因，例如BAALC、WT1、MN1、MEF2C、LMO1与LMO2。LOUCY细胞中排名前500的增强子区域相关基因，在JQ1处理后下调的基因中显著富集。本实验将LOUCY细胞以250nM的(+)-JQ1或二甲基亚砜（dimethyl sulfoxide, DMSO）处理4小时，每组均设置3次生物学重复。