Size group (pico, nano, micro) and group total carbon estimates from cell counts via epifluorescent microscopy (EPI) of heterotrophic and autotrophic plankton from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2011 (ongoing).

Microbial community assemblages of the California Current Ecosystem (CCE) are assessed for biomass of heterotrophic (dinoflagellate and other eukaryotes) and autotrophic (dinoflagellate and other eukaryotes) plankton using high-throughput digital epifluorescence microscopy (EPI). Samples to estimate the nano- and microplankton (0.2-2.0-µm and 2.0-20-µm size, respectively) are collected at various depths via Niskin bottles, preserved, stained, and filtered onto a membrane filter and mounted on a glass microscope slide aboard the quarterly CalCOFI survey cruises (since 2004, ongoing). Slides are then frozen at -80°C for subsequent imaging and analysis in the laboratory onshore. Carbon biomass is computed from cell biovolumes.

本数据集针对加州流生态系统（California Current Ecosystem, CCE）的微生物群落集合体，采用高通量数字落射荧光显微镜（high-throughput digital epifluorescence microscopy, EPI），对异养型（heterotrophic，涵盖甲藻dinoflagellate及其他真核生物eukaryotes）与自养型（autotrophic，涵盖甲藻及其他真核生物）浮游生物plankton的生物量开展评估。
用于估算纳米浮游生物（粒径0.2~2.0μm）与微型浮游生物（粒径2.0~20μm）的样本，通过尼斯金采水器（Niskin bottles）在不同深度采集后，于自2004年起持续开展的季度性CalCOFI调查航次的船上完成固定、染色、过滤至滤膜并贴附于载玻片的操作。
随后将载玻片置于-80℃环境中冷冻保存，用于后续岸上实验室的成像与分析工作。
碳生物量通过细胞生物体积计算得到。