Dataset related to article "CRISPR Genome-Wide screening reveals chemosensor receptors as lipid-detecting regulators of macrophage functions in cancer"

This record contains raw data related to article "CRISPR Genome-Wide screening reveals chemosensor receptors as lipid-detecting regulators of macrophage functions in cancer" Abstract: The infiltration of macrophages is considered a hallmark of cancer progression and re-education of tumor-associated macrophages towards an anti-tumour status is considered a promising strategy of cancer immunotherapy. However, the mechanisms governing macrophage education by cancer cells remain largely elusive, posing challenges for therapeutic exploitation. To gain deeper insights into TAMs' plasticity, we conducted an unbiased genome-wide CRISPR screening on primary macrophages. Our investigation confirmed the role of known regulators, including Stat6, in orchestrating TAMs' responses and unveiled novel insights into TAMs' behaviour. We identified olfactory and vomeronasal receptors, commonly referred to as chemosensors, as major drivers of macrophages tumor-supportive status in multiple cancers. Collectively, our findings implicate chemosensors in macrophage sensing of the lipid-enriched milieu and highlight these receptors as therapeutic targets for enhancing antitumor immunity.本数据集收录了与论文《CRISPR全基因组筛选揭示化学感受器受体作为癌症中巨噬细胞功能的脂质感知调控因子》相关的原始实验数据。 摘要： 巨噬细胞浸润被视作癌症进展的标志性特征，将肿瘤相关巨噬细胞（tumor-associated macrophages, TAMs）重教育为抗肿瘤表型，被认为是癌症免疫治疗的极具潜力的策略。然而，癌细胞调控巨噬细胞重教育的分子机制在很大程度上仍不明确，这为治疗应用带来了诸多挑战。 为深入探究肿瘤相关巨噬细胞的可塑性，我们对原代巨噬细胞（primary macrophages）开展了无偏倚CRISPR全基因组筛选。本研究验证了包括信号转导与转录激活因子6（Stat6）在内的已知调控因子在调控肿瘤相关巨噬细胞应答过程中的作用，并揭示了肿瘤相关巨噬细胞行为的全新见解。我们鉴定出嗅觉受体与犁鼻器受体，这类受体通常被称为化学感受器（chemosensor），是多种癌症中巨噬细胞形成肿瘤支持表型的主要驱动因子。综上，本研究结果表明化学感受器参与巨噬细胞对富含脂质的肿瘤微环境的感知，并凸显这类受体作为增强抗肿瘤免疫的治疗靶点的潜在价值。