Juvenile hormone regulates the maturation of sexually dimorphic naïve ethanol olfactory preference in Drosophila melanogaster

We have identified a sexually dimorphic behavior in adult Drosophila melanogaster that varies depending on age, sex, and mating status. Our results suggest that the rewarding neuropeptide Corazonin (Crz), the transcription factor Apontic, and juvenile hormone (JH) signaling regulate naïve ethanol olfactory preference (NEOP) responses during early stages of adulthood. Pharmacological blockade of JH via precocene increases NEOP in females and males, which is phenocopied by the knockdown of JH receptors Met and Gce globally in the nervous system, as well as specifically in the mushroom body. This suggests a novel role of JH in dimorphic ethanol behaviors. Mating decreases NEOP in males, likely acting through rewarding pathways such as Neuropeptide F (NPF) and Crz. Our study suggests an early evolutionary emergence of hormonal mechanisms regulating ethanol-dependent behaviors, as Crz and JH share similarities with the vertebrate gonadotropin-releasing hormone (GnRH) and thyroid hormones, re..., , , # Juvenile hormone regulates the maturation of sexually dimorphic naïve ethanol olfactory preference in Drosophila melanogaster

The dataset consists of the following files: genotypeNEOPdata.csv, NEOP.PREC.csv, and NEOPdatafig1no1.csv contain data collected from each T-maze trial of ~10 flies.

Each fly had the option to choose the ethanol chamber, the control chamber, or neither (staying in the starting chamber). These data were tallied as Experimental Stimulus, Control Stimulus, and No response, respectively. \"Batch\" refers to the specific maze being recorded, but was nested within the date for analysis. Each file has different column headers to denote the manipulation being used for the specific experiment. For example, genotypeNEOPdata.csv only with the inins genotype, while NEOP.PREC.csv also contains \"Prec,\" which is the precocene concentration that animals were treated within that trial.

**Variable Key**:

* Batch: An ID number for each test

- Date: The date on which that test ...,

我们在黑腹果蝇（Drosophila melanogaster）成虫中发现了一种性二态行为，该行为会随年龄、性别以及交配状态发生变化。我们的研究结果显示，奖赏性神经肽Corazonin (Crz)、转录因子Apontic以及保幼激素（juvenile hormone, JH）信号通路，可在成虫早期阶段调控初次乙醇嗅觉偏好（naïve ethanol olfactory preference, NEOP）应答。通过早熟素（precocene）对JH进行药理学阻断，可使雌雄果蝇的NEOP水平升高；在神经系统中全局敲低JH受体Met与Gce，或是仅在蕈形体（mushroom body）中进行该操作，均可复刻这一表型。这表明JH在乙醇相关的性二态行为中具有全新的调控功能。交配会降低雄性果蝇的NEOP水平，这一过程可能通过神经肽F（Neuropeptide F, NPF）与Crz等奖赏通路实现。本研究提示，调控乙醇依赖行为的激素调控机制可能早在演化早期就已出现——因为Crz与JH分别与脊椎动物的促性腺激素释放激素（gonadotropin-releasing hormone, GnRH）和甲状腺激素具有相似性。
# 保幼激素调控黑腹果蝇性二态初次乙醇嗅觉偏好的成熟过程

本数据集包含以下文件：genotypeNEOPdata.csv、NEOP.PREC.csv与NEOPdatafig1no1.csv均包含约10只果蝇参与的T迷宫试验采集得到的数据。每只果蝇可选择乙醇迷宫室、对照迷宫室，或是两者都不进入（停留在起始室）。对应的数据分别被记为实验刺激组、对照刺激组与无响应组。“批次（Batch）”指本次记录的特定迷宫，但在分析时需嵌套于日期维度。每个文件的列标题各不相同，用于标识对应实验所采用的处理方式。例如，genotypeNEOPdata.csv仅包含inins基因型的相关数据，而NEOP.PREC.csv还包含“Prec”字段，即该试验中受试动物所接受的早熟素浓度。

**变量说明**：
* 批次（Batch）：每项测试的唯一标识编号
- 测试日期（Date）：该测试的执行日期……