Encompassing view of spatial and single-cell RNA-seq renews the role of the microvasculature in human atherosclerosis

Here we generate an integrative high-resolution map of human atherosclerotic plaques combining single-cell RNA-seq from multiple studies and spatial transcriptomics data from 12 human specimens, with different stages of atherosclerosis. We show cell-type and atherosclerosis-specific expression changes and spatially constrained alterations in cell-cell communication.本研究整合多项研究的单细胞RNA测序（single-cell RNA-seq）数据，以及12例不同分期动脉粥样硬化人体标本的空间转录组学（spatial transcriptomics）数据，构建了人类动脉粥样硬化斑块的高分辨率整合图谱。本研究揭示了细胞类型特异性及动脉粥样硬化特异性的基因表达变化，以及细胞间通讯的空间受限改变。