Data_Sheet_1_Origanum majorana L.: A Nutritional Supplement With Immunomodulatory Effects.ZIP

Origanum majorana L. is an aromatic herb that has been grown in several Mediterranean countries since ancient times, but became popular during the Middle Ages as a medicinal plant and seasoning ingredient. O. majorana has many pharmacological effects, but its immunoreactive components and mechanisms are still unclear. In this study, four compounds were isolated and identified from O. majorana by a spectral analysis, including 1H and 13C-NMR. They were 1H-indole-2-carboxylic acid (1), (+)-laricresol (2), (+)-isolaricresol (3), and procumboside B (4, pB), which were isolated for the first time in O. majorana. The immunomodulatory effects of the four compounds were screened, and pB had good immunomodulatory activity on RAW 264.7 cells. The immunomodulatory mechanism of pB was proved, in which pB could increase the secretion of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and reactive oxygen species (ROS) and simultaneously upregulate the expression of CD80 and CD86 on the cell surface. These results suggested that the mechanism of pB may be related to the activation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs)-signaling pathways. O. majorana is rich in nutrients and is commonly used in diets, so it can be used as a nutritional supplement with immunomodulatory effects.马郁兰（Origanum majorana L.）是一种芳香草本植物，自上古时期便在多个地中海国家广为种植，于中世纪作为药用植物与调味原料广受青睐。马郁兰具备多种药理活性，但其免疫活性成分与作用机制仍未明确。本研究通过光谱分析技术（涵盖1H与13C核磁共振波谱法）从马郁兰中分离鉴定出4种化合物，分别为1H-吲哚-2-羧酸（1）、(+)-落叶松脂醇（(+)-laricresol）、(+)-异落叶松脂醇（(+)-isolaricresol）以及铺地糖苷B（procumboside B，4，简称pB），上述化合物均为首次在马郁兰中被分离得到。研究团队对这4种化合物的免疫调节活性开展了筛选，结果显示procumboside B（简称pB）对RAW 264.7细胞展现出良好的免疫调节活性。本研究进一步阐明了pB的免疫调节机制：pB可促进一氧化氮（nitric oxide, NO）、白细胞介素-6（interleukin-6, IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）与活性氧（reactive oxygen species, ROS）的分泌，同时上调细胞表面CD80与CD86的表达水平。上述结果表明，pB的作用机制可能与核因子-κB（nuclear factor-kappaB, NF-κB）及丝裂原活化蛋白激酶（mitogen-activated protein kinases, MAPKs）信号通路的激活密切相关。马郁兰营养丰富且常被应用于膳食当中，因此可作为具备免疫调节功效的营养补充剂进行开发与利用。