Transcriptome analysis of gene expression in sec59-1∆ yeast cells. Saccharomyces cerevisiae

Microarray data revealed that, 1880 genes were up regulated and 1430 genes were down regulated among that 3310 genes, 92 lipidomic genes were up regulated and 17 genes were down regulated in sec59-1∆. In sec59-1∆ cells, phospholipid, neutral lipid, sphingolipid and fatty acid metabolism genes were up regulated. N-Glycosylation defect affected various organelle functions such as mitochondria, peroxisome, vacuole and ER. Finally we can conclude from the microarray data, N-glycosylation plays a crucial role in regulating the lipidome homeostasis Microarray data revealed that, 1880 genes were up regulated and 1430 genes were down regulated among that 3310 genes, 92 lipidomic genes were up regulated and 17 genes were down regulated in sec59-1∆. In sec59-1∆ cells, phospholipid, neutral lipid, sphingolipid and fatty acid metabolism genes were up regulated. N-Glycosylation defect affected various organelle functions such as mitochondria, peroxisome, vacuole and ER. Finally we can conclude from the microarray data, N-glycosylation plays a crucial role in regulating the lipidome homeostasis Overall design: Organism : Saccharomyces cerevisiae , Custom Saccharomyces cerevisiae 8x15k Gene expresssion Microarray (AMADID: 016333) designed by Genotypic Technology Private Limited基因芯片（Microarray）分析结果显示：在3310个检测基因中，sec59-1∆菌株内有1880个基因上调表达、1430个基因下调表达；其中脂质组相关基因有92个上调、17个下调。在sec59-1∆细胞中，磷脂、中性脂、鞘脂及脂肪酸代谢相关基因均呈上调表达状态。N-糖基化（N-Glycosylation）缺陷会影响线粒体、过氧化物酶体、液泡及内质网（ER）等多种细胞器的功能。最终通过基因芯片数据分析可得出结论：N-糖基化在调控脂质组稳态中发挥关键作用。 实验设计概述： 实验生物：酿酒酵母（Saccharomyces cerevisiae） 采用由Genotypic Technology Private Limited公司设计的定制化酿酒酵母8x15k基因表达基因芯片（AMADID: 016333）