Single Cell sequencing analysis of AT1/AT2 cells from wildtype mouse, AT1-Cdc42 knockout mouse, and left bronchial ligation model. Single Cell sequencing analysis of AT1/AT2 cells from wildtype mouse, AT1-Cdc42 knockout mouse, and left bronchial ligation model

The goal of this study was to compare wildtype adult lung epithelium to epithelium from Cdc42 AT1-KO mice and mice after left bronchial ligation model. Overall design: Two samples of adult mouse epithelial cells, three samples of adult Hopx-CreERT2; Cdc42 KO lineage traced mice, and three samples of Hopx-CreERT2 lineage traced mice after left bronchial ligation were pooled respectively for library preparation.

本研究旨在对比野生型（wildtype）成年肺上皮组织，与Cdc42 AT1基因敲除（Cdc42 AT1-KO）小鼠及左支气管结扎模型（left bronchial ligation model）小鼠的上皮组织。实验整体设计如下：分别将2份成年小鼠上皮细胞样本、3份成年Hopx-CreERT2；Cdc42基因敲除谱系示踪小鼠样本，以及3份经左支气管结扎后的Hopx-CreERT2谱系示踪小鼠样本混合，用于文库制备。