Additional file 1: of ErbB4 promotes malignant peripheral nerve sheath tumor pathogenesis via Ras-independent mechanisms

Figure S1. ErbB4 lysates are sensitive to denaturing detergents. Table S1. Patient demographics for immunostained MPNSTs. Figure S2 Real-time PCR analyses of erbB4 splice variants and mouse MPNST erbB4 expression. Table S2. MPNST locations in P0-GGFβ3;Trp53+/−;Erbb4flox/flox mice. Figure S3. Validation of Erbb4 ablation and effects on expression of other erbB receptors. Figure S4. Kinase array blots for NRG1β and unstimulated control and Erbb4-null MPNST cells. Figure S5. Downregulation of WNK1 expression did not affect cell viability. (ZIP 7528 kb)

图S1：ErbB4细胞裂解液对变性去污剂敏感。
表S1：免疫染色的恶性周围神经鞘膜瘤（Malignant Peripheral Nerve Sheath Tumor，MPNST）患者人口统计学特征。
图S2：erbB4剪接变体及小鼠MPNST中erbB4表达的实时PCR分析。
表S2：P0-GGFβ3；Trp53+/-；Erbb4flox/flox 基因工程小鼠的MPNST发生部位。
图S3：Erbb4基因敲除验证及对其他erbB受体表达的影响。
图S4：NRG1β刺激组、未刺激对照组及Erbb4敲除MPNST细胞的激酶阵列印迹。
图S5：WNK1表达下调对细胞活力无影响。
（压缩包大小7528千字节）