scRNA-seq of brain glioblastoma sample. scRNA-seq of brain glioblastoma sample

Proneural-mesenchymal transition (PMT) is a phenotypic alteration and contributes to therapeutic resistance and recurrence of glioblastoma (GBM). Macrophages, as a main infiltrating component of tumor immune microenvironment (TIM), can regulate the biological processes of PMT. However, the mechanisms driving this process remain largely unknown. Here, We performed single-cell RNA sequencing (scRNA-seq) (3V3) and Spatial transcriptomics RNA sequencing(stRNA-seq)(1V1) from tumor core and matching tumor periphery samples to discripe the overall landscape of tumor and nontumor cells in gliomas. Overall design: We profiled freshly isolated cells from tumor core and paired surrounding peripheral tissue in glioma patients. scRNA-seq (3V3) and stRNA-seq(1v1) were used to discripe the overall landscape of tumor and nontumor cells in gliomas.神经前体-间质转化（Proneural-mesenchymal transition, PMT）是一种表型改变，可促进胶质母细胞瘤（glioblastoma, GBM）的治疗抵抗与复发。巨噬细胞作为肿瘤免疫微环境（tumor immune microenvironment, TIM）的主要浸润组分，能够调控PMT的生物学进程，但目前该过程的驱动机制仍尚不明确。 本研究从胶质瘤患者的肿瘤核心区域及匹配的肿瘤外周组织样本中获取材料，开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）(3V3)与空间转录组RNA测序（Spatial transcriptomics RNA sequencing, stRNA-seq）(1V1)，以刻画胶质瘤内肿瘤细胞与非肿瘤细胞的整体转录组景观。 实验设计概述：本研究对胶质瘤患者的肿瘤核心区域及配对的外周邻近组织中的新鲜分离细胞进行了转录组分析，采用单细胞RNA测序（scRNA-seq）(3V3)与空间转录组RNA测序（stRNA-seq）(1V1)技术，描绘胶质瘤中肿瘤细胞与非肿瘤细胞的整体转录组景观。