Transcriptome analysis of the venom gland of Centruroides hirsutipalpus. Transcriptome analysis of the venom gland of Centruroides hirsutipalpus by RNA-seq

Venom glands from the telsons of four specimens of the Mexican scorpion Centruroides hirsutipalpus consisting in 2 males and 2 females were dissected and the total RNA was extracted. A cDNA library was constructed and subjected to RNA-seq by Illumina. The reads were assembled de novo with Trinity and annotated with Trinotate. A total of 2,553,529 reads were obtained by the RNA-seq procedure. The Trinity assembly resulted in a total of 44,579 transcripts. After bioinformatic analysis, 141 transcripts were identified potentially coding for scorpion venom components.

研究者对2雄2雌共4头墨西哥蝎（Centruroides hirsutipalpus）标本的尾节（telson）毒腺进行解剖分离，随后提取总RNA并构建cDNA文库，采用Illumina平台开展RNA测序（RNA-seq）。测序所得读段（reads）使用Trinity软件进行从头转录组组装，并通过Trinotate完成功能注释。本次RNA测序流程共获得2,553,529条读段，经Trinity组装后得到44,579条转录本（transcripts）；经生物信息学分析后，共鉴定出141条可能编码蝎毒相关成分的转录本。