Bidirectional histone monoaminylation dynamics regulate neural rhythmicity

H3 monoaminylation dynamics are bidirectionally controlled by TG2 and facilitate neural rhythmicity. Overall design: CUT&RUN experiments across the ZT in the mouse TMN include H3K4me2, H3K4me3, H3K4me3Q5ser, H3K4me3Q5his, and WDR5. CUT&RUN in HeLa cells including H3K4me3Q5his and H3Q5his.组蛋白H3单胺酰化修饰的动力学过程受转谷氨酰胺酶2（Transglutaminase 2, TG2）双向调控，并可促进神经节律性。实验总体设计：在小鼠结节乳头核（Tuberomammillary Nucleus, TMN）的不同授时因子时间（Zeitgeber Time, ZT）阶段开展的CUT&RUN实验，检测靶标包括H3K4me2、H3K4me3、H3K4me3Q5ser、H3K4me3Q5his以及WD重复蛋白5（WDR5）；此外在HeLa细胞中开展的CUT&RUN实验则检测了H3K4me3Q5his与H3Q5his。