N-glycosylation analysis of human complement component C3 LC-MS

The most abundant and central component, glycoprotein C3, contributes to the development of type 1 diabetes by enhancing the organ-specific autoimmune inflammatory processes. It is known that changes in glycosylation can modulate inflammatory responses and we recently showed that children at the onset of type 1 diabetes have a higher proportion of oligomannose glycans in plasma N-glycome compared to their healthy siblings. Due to fact that C3 contains two N-glycosylation sites occupied by this type of glycans, our aim was to develop a novel high-throughput and cost-effective glycoproteomic workflow for N-glycosylation analysis of human C3 to reveal the possible role of C3 glycosylation in type 1 diabetes development.

作为体内含量最为丰富且核心的组分，糖蛋白C3（glycoprotein C3）可通过增强器官特异性自身免疫炎症进程，参与1型糖尿病的发生发展。已知糖基化改变可调控炎症应答，本团队近期研究发现，与健康同胞相比，初发1型糖尿病患儿的血浆N-糖组（N-glycome）中高甘露糖型聚糖（oligomannose glycans）的占比更高。鉴于C3含有两个被该类聚糖修饰的N-糖基化（N-glycosylation）位点，本研究旨在开发一种新型高通量且成本效益高的糖蛋白质组学工作流程，用于人源C3的N-糖基化分析，以揭示C3糖基化在1型糖尿病发生发展中可能发挥的作用。