In situ hybridization of Xenopus laevis with chordin and actc1 probe after knockdown of MMP3, Noggin2 and Chordin genes.

This dataset demonstrates the influence of MMP3 on the development of mesoderm tissues in Xenopus laevis embryos. Chordal mesoderm is visualized with Chordin probe, whereas somite mesoderm - with Actc1 probe. All embryos were fixed at stage 15 (NF). Our hypothesis considers MMP3 to stabilize the Chordin, and the former degrade faster under low MMP3 level that follows in smaller somite mesoderm. At the same time, MMP3 increases the degradation of Noggin2, which is expressed in the organizer region and expands the area of chordal mesoderm.

本数据集展示了基质金属蛋白酶3（MMP3）对非洲爪蟾（Xenopus laevis）胚胎中胚层组织发育的影响。脊索中胚层采用脊索蛋白（Chordin）探针进行可视化标记，体节中胚层则通过Actc1探针完成可视化。所有胚胎均于NF分期第15期固定。
本研究假说认为：MMP3可稳定脊索蛋白（Chordin）；当MMP3水平降低时，脊索蛋白降解速率加快，进而导致体节中胚层体积减小。与此同时，MMP3可促进头蛋白2（Noggin2）的降解；头蛋白2在胚胎组织者区域表达，能够扩大脊索中胚层的分布范围。