Bulk RNAseq analysis of hypertrophic cardiomyopathy (HCM) hiPSC-derived cardiomyocytes (hiPSC-CMs) and the isogenic control

To investigate HCM mutation-associated molecular details at the early stage of disease development, we performed bulk RNA-seq analysis at Day 15 of differentiation using RNA isolated from the isogenic control and mutant hiPSC-CMs. We performed pair-end sequencing with >30 million reads per sample with clean read counts of more than 96% for each sample. mRNA profiles of Day15 MYH7 R723C with MYH6 R725C mutant hiPSC-CMs and isogenic control

为探究疾病发生早期与肥厚型心肌病（HCM）相关突变的分子细节，本研究利用分离自同基因对照及突变型诱导多能干细胞来源心肌细胞（hiPSC-CMs）的RNA，在细胞分化第15天开展了批量RNA测序（bulk RNA-seq）分析。本研究对所有样本均实施双端测序，单样本读段数超过3000万，且各样本的清洁读段占比均高于96%。本研究同时分析了分化第15天携带肌球蛋白重链7（MYH7）R723C联合肌球蛋白重链6（MYH6）R725C突变的hiPSC-CMs及其同基因对照的mRNA表达谱。