SUM159 cells grown in 2D cultures in the presence or absence of SHP2

The first bona fide PTP proto-oncogene was the Src-homology 2 domain-containing phosphatase SHP2 (encoded by PTPN11), an ubiquitously expressed PTP that transduces mitogenic, pro-survival, cell fate and/or pro-migratory signals from numerous growth factor-, cytokine- and extracellular matrix receptors. In malignancies, SHP2 is hyperactivated either downstream of oncoproteins or by mutations.We provide analysis of the human mesenchymal triple-negative breast cancer cell line SUM159 and grown in 2D cultures for 15 days in the presence or absence of SHP2. The human mesenchymal triple-negative breast cancer cells SUM159 were transduced with a doxycycline-inducible lentiviral vector expressing a CTRL miR or SHP2 miR1 or SHP2 miR2. Cells from each group were grown in 2D cultures, and the knockdown of SHP2 was induced for 15 days. RNA was extracted for gene expression analysis.首个真正意义上的蛋白酪氨酸磷酸酶（protein tyrosine phosphatase, PTP）原癌基因，是由PTPN11基因编码的含Src同源2（Src Homology 2, SH2）结构域的磷酸酶SHP2（Src-homology 2 domain-containing phosphatase SHP2）。该酶为广泛表达的PTP，可转导来自多种生长因子、细胞因子及细胞外基质受体的促有丝分裂、促存活、细胞命运调控以及促迁移信号。在恶性肿瘤中，SHP2可通过癌蛋白下游通路激活或发生自身突变而被过度活化。本数据集包含人间质型三阴性乳腺癌细胞系SUM159的相关分析数据：该细胞系分别在存在SHP2或缺失SHP2的条件下，于二维（2D）培养体系中培养15天。将SUM159人间质型三阴性乳腺癌细胞转导至多西环素诱导型慢病毒载体（doxycycline-inducible lentiviral vector），该载体可表达对照微RNA（CTRL miR）、SHP2靶向微RNA1（SHP2 miR1）或SHP2靶向微RNA2（SHP2 miR2）。各组细胞均置于二维培养体系中培养，并诱导SHP2基因敲低达15天，随后提取细胞总RNA用于基因表达分析。