Single-molecule sequencing of the Drosophila serrata genome. Drosophila serrata strain:Fors4

Here we sequence and de novo assemble the genome of Drosophila serrata, a non-model species from the montium subgroup that has been well studied for clines and sexual selection. Using 11 PacBio SMRT cells, we generated 12 Gbp of raw sequence data comprising approximately 65x whole genome coverage. Read lengths averaged 8,940 bp (NRead50 12,200) with the longest read at 53 Kbp. We self-corrected reads using the PBDagCon algorithm and assembled the genome using the MHAP algorithm within the PBcR assembler. Total genome length was 198 Mbp with an N50 just under 1 Mbp. Contigs displayed a high degree of arm-level conservation with D. melanogaster. We also provide an initial annotation for this genome using in silico gene predictions that were supported by RNA-seq data.

本研究对棘果蝇（Drosophila serrata）的基因组进行测序并完成从头组装，该物种隶属于山果蝇亚组（montium subgroup），是一类因渐变群与性选择研究而被广泛探究的非模式物种。本研究使用11个PacBio单分子实时测序（SMRT）单元，产出总计12 Gbp的原始测序数据，全基因组覆盖度约为65倍。测序读长的平均长度为8940 bp，读长NRead50为12200 bp，最长读长可达53 Kbp。研究团队采用PBDagCon算法对测序读长进行自我校正，并借助PBcR组装软件内置的MHAP算法完成基因组组装。组装获得的基因组总长度为198 Mbp，基因组N50略小于1 Mbp。该基因组的重叠群（contigs）与黑腹果蝇（D. melanogaster）在染色体臂水平上呈现高度保守性。本研究同时基于经RNA测序（RNA-seq）数据验证的计算机模拟基因预测结果，为该基因组提供了初步注释。