Determination of Shavenbaby target genes in Enteblast (EBs)

Shavenbaby (Svb) transcription factor is involved in the homeostasis of intestinal stem cells (ISCs), and the differentiation of their enteroblast progeny (EB) into enterocytes (EC). Svb is produced as a long repressor protein called SvbRepressor (SvbREP). In presence of Pri peptides SvbREP is processed into a shorter activator form (SvbACT) through partial proteasomal degradation of the REP domain. In ISCs/EBs, Pri triggers SvbREP to ACT maturation, while SvbREP form accumulates in differentiated enterocytes. The absence of Svb promote the apoptosis of the intestinal stem cells. In ISCs/EBs, the ectopic expression of SvbREP form promotes the differentiation and the overexpression of SvbACT promotes hyperplasia. (Al Hayek, et al 2020) Based on these functional analyses, Svb appears to control ISC/EB survival and behavior, balancing proliferation and differentiation through a molecular switch between its 2 antagonistic isoforms. In this study, we determine the Svb target genes in different cell types along the intestinal cell lineage. This particular metadata sheet refers to enteroblast (EB) transcriptomic data (RNAseq) in control condition and upon modulation of Svb function. The samples correspond to control (EB Ctrl), Svb-knock down EBs (EB RNAiSvb ), SvbACT constitutive form overexpression (EB SvbAct) and SvbREP constitutive isoform overexpression (EB SvbRep). Three biological replicates were performed for each genotype, on different calendar days.Shavenbaby (Svb) 转录因子（transcription factor）参与肠道干细胞（intestinal stem cells, ISCs）的稳态维持，并调控其成肠细胞子代（enteroblast progeny, EB）向肠上皮细胞（enterocytes, EC）的分化过程。Svb最初以长链阻遏蛋白形式合成，该形式被命名为Svb阻遏蛋白（SvbRepressor, SvbREP）。当存在Pri肽时，SvbREP会通过REP结构域的部分蛋白酶体降解，被加工为较短的激活型形式（SvbACT）。在肠道干细胞/成肠细胞中，Pri可介导SvbREP向激活型的成熟转化，而SvbREP形式则会在已分化的肠上皮细胞中积累。Svb的缺失会促进肠道干细胞的凋亡。在肠道干细胞/成肠细胞中，异位表达SvbREP会促进细胞分化，而过表达SvbACT则会引发细胞增生过度。（Al Hayek等，2020）基于上述功能分析结果，Svb似乎通过其两种拮抗亚型间的分子开关，调控肠道干细胞/成肠细胞的存活与行为，平衡细胞增殖与分化进程。本研究旨在明确肠道细胞谱系中不同细胞类型内的Svb靶基因。本元数据表对应的是对照条件下以及Svb功能调控后的成肠细胞（EB）转录组测序（RNAseq）数据。样本分为以下四组：对照组成肠细胞（EB Ctrl）、Svb敲低成肠细胞（EB RNAiSvb）、组成型过表达SvbACT的成肠细胞（EB SvbAct），以及组成型过表达SvbREP亚型的成肠细胞（EB SvbRep）。每种基因型均设置3次生物学重复，实验于不同日历日期开展。