RNA-seq of mouse fetal intestines comparing wildtype littermates with Notch inhibited mutants

Whole intestines were harvested from fetuses from timed pregnant matings for wildtype littermates and endothelial specific Notch inhibited mutants (Jax strain #032613). Fetal stages were confirmed according to the Theiler staging chart (https://www.emouseatlas.org/emap/ema/staging_criteria/staging_criteria.html). Whole intestines (from the common bile duct through the cecum) were collected at E16-E16.5. Male and female intestines from each stage were pooled and dissociated to single cells for single cell RNA sequencing as previously described (Miller et al. 2020 Dev Cell). 705-KW-1 and 705-KW-2 were whole intestine. 2482-KW1 and 2482-KW2 samples were enriched with CD31+ MACS beads. 3593-KW1 and 3593-KW2 samples were enriched with CD31+ MACS beads and depleted for CD45.