Soil arbuscular mycorrhizal fungi in New Zealand grassland as affected by plant-soil feedbacks and simulated fire

This study explored plant-soil feedbacks and heat to simulate fire on mutualistic arbuscular mycorrhizal (AM) fungal communities and invasive plant growth. Soils were collected from a dominant native grass (Chionochloa macra) and two invasive hawkweeds (Hieracium lepidulum, Pilosella officinarum) in a New Zealand grassland, then heated to 30, 45 and 60 degres Celsius.AM fungal communities were targeted sequencing the 18S rRNA or SSU with primers WANDA/AML2 and sequenced with Illumina MiSeq PE300. Only forward sequences were analyzed and are uploaded here.

本研究针对互惠共生丛枝菌根（arbuscular mycorrhizal，AM）真菌群落与入侵植物生长，探究了植物-土壤反馈以及热模拟火灾的作用效应。实验土壤采集自新西兰草原的优势本土禾草大寒原草（Chionochloa macra）以及两种入侵山柳菊——淡色山柳菊（Hieracium lepidulum）与匍枝山柳菊（Pilosella officinarum）的生境，随后将土壤分别加热至30、45与60摄氏度。针对丛枝菌根真菌群落，研究采用引物WANDA/AML2对18S rRNA（即小亚基核糖体RNA，SSU）区域进行靶向测序，测序使用Illumina MiSeq PE300平台完成。本研究仅对正向序列开展分析，相关数据已上传至本次公开数据集。