Circular extrachromosomal DNA promotes accessible chromatin and high oncogene expression

The pixel intensity quantification of ATAC-see confocal images. It is used for creating the Extended Data Figure 7d of the manuscript entitled "Circular extrachromosomal DNA promotes accessible chromatin and high oncogene expression" . The brief ATAC-see method for metaphase chromosome spread is as follow: metaphase sample was prepared onto a 1-mm coverslip and incubated with 50 nM of ATTO-590 transposome under 37°C for 30 min in the dark. After washed twice by 2× SSC with 0.01% SDS for 15 min, and once by 2× SSC with 0.2% Tween-20 for 15 min, sample was subjected to FISH procedures, and finally stained by 1 µg/ml DAPI and mount with VECTASHIELD antifade mounting media (Vector Laboratories).<br>

本数据集为ATAC-see共聚焦显微镜图像的像素强度定量结果，用于支撑题为《环状染色体外DNA促进开放染色质与高癌基因表达》的学术论文的补充数据图7d（Extended Data Figure 7d）。
针对中期染色体铺片的ATAC-see简要实验流程如下：将中期样品铺展于1mm盖玻片上，于37℃避光环境中用50 nM的ATTO-590转座体（ATTO-590 transposome）孵育30分钟。随后依次使用含0.01%十二烷基硫酸钠（SDS）的2×柠檬酸钠盐缓冲液（2× SSC）洗涤两次，每次15分钟；再使用含0.2%吐温-20（Tween-20）的2× SSC洗涤一次，时长15分钟。之后对样品开展荧光原位杂交（FISH）实验流程，最终以1 µg/ml的4',6-二脒基-2-苯基吲哚（DAPI）进行染色，并采用VECTASHIELD抗荧光淬灭封片剂（Vector Laboratories）完成封片。