Additional file 1: of Rat limbal niche cells can induce transdifferentiation of oral mucosal epithelial cells into corneal epithelial-like cells in vitro

S1. The materials used in cell isolation and culture. S2. The primer sequences used in RT-PCR. S3. The antibodies used in the experiments. S4. PCR of DF-LNC and ME-LNC. S5. Western blot of DF-LNC and ME-LNC. S6. PCR of 3D cocultured OMECs and LNCs. S7. Cell counting of 3D cocultured OMECs and LNCs. S8. PCR of Transwell cultured groups. S9. Western blot of Transwell cultured groups. S10. PCR of 3T3 cells and renewed LNCs cultured in Transwell. S11. Western blot of 3T3 cells and renewed LNCs cultured in Transwell. S12. Cocultured OMECs on APCS. S13. IACUC. (ZIP 6676 kb)S1. 细胞分离与培养实验所用材料 S2. 逆转录聚合酶链式反应（Reverse Transcription-Polymerase Chain Reaction，RT-PCR）所用引物序列 S3. 实验所用抗体 S4. DF-LNC与ME-LNC的聚合酶链式反应（Polymerase Chain Reaction，PCR）实验 S5. DF-LNC与ME-LNC的蛋白质免疫印迹（Western Blot）实验 S6. 三维共培养的OMECs与LNCs的PCR实验 S7. 三维共培养的OMECs与LNCs的细胞计数实验 S8. Transwell培养组的PCR实验 S9. Transwell培养组的蛋白质免疫印迹（Western Blot）实验 S10. Transwell培养体系中培养的3T3细胞与更新后LNCs的PCR实验 S11. Transwell培养体系中培养的3T3细胞与更新后LNCs的蛋白质免疫印迹（Western Blot）实验 S12. APCS载体上的共培养OMECs S13. 实验动物伦理审查委员会（Institutional Animal Care and Use Committee，IACUC）相关材料 （压缩包大小：6676 KB）