Prevalence of Bd infection across amphibian communities and populations.

aLICA only are wetlands where we detected only L. catesbeianus populations; co-occurring wetlands are those where we detected the presence of both L. catesbeianus populations and at least one other native amphibian population, native only are wetlands where we detected populations of at least one native amphibian species but no L. catesbeianus individuals. bWetlands were designated Bd+ if at least one sample collected from the wetland tested positive for Bd. The site level prevalence is the proportion of sampled wetlands of each wetland community type with at least one individual that tested positive for Bd. cThe species level prevalence is the proportion of sampled individuals of each species that tested positive for the pathogen Bd at each of the different wetland community types. Prevalence of Bd infection determined from all amphibian populations at the 36 wetlands in the three different wetland community types from which we collected population level Bd data. The North American bullfrog (L. catesbeianus) was the only non-native amphibian species encountered, while tiger salamanders (A. tigrinum), Woodhouse’s toads (A. woodhousii), and western chorus frogs (P. triseriata) are all native amphibian species in the Colorado study system. We were able to obtain population-level Bd estimates data from only one population of Northern leopard frogs (L. pipiens), and therefore we did not include this species into our analysis. See Table S3 in File S1 for further detail about Bd infection in L. pipiens in our study. Prevalence of Bd infection across amphibian communities and populations.a. 仅美洲牛蛙（L. catesbeianus）湿地指仅检测到美洲牛蛙（L. catesbeianus）种群的湿地；共分布湿地指同时检测到美洲牛蛙种群及至少一种其他本土两栖动物种群的湿地；仅本土物种湿地指检测到至少一种本土两栖动物物种种群但未检测到美洲牛蛙个体的湿地。 b. 若某湿地采集的至少一份样本检测结果呈蛙壶菌（Bd）阳性，则将该湿地归类为Bd阳性湿地。样点水平感染率指每类湿地群落类型中，至少有一个个体呈Bd阳性的采样湿地占比。 c. 物种水平感染率指在不同湿地群落类型中，各物种种群的采样个体中呈Bd病原体阳性的个体占比。 本研究基于收集了种群水平Bd检测数据的3类不同湿地群落类型下的36处湿地的所有两栖动物种群，统计得到Bd感染率。本研究仅发现美洲牛蛙（L. catesbeianus）这一种外来两栖动物物种，而虎钝口螈（A. tigrinum）、伍德豪斯蟾蜍（A. woodhousii）以及西部鸣蛙（P. triseriata）均为科罗拉多研究区域内的本土两栖动物物种。我们仅获得了北部豹蛙（L. pipiens）一个种群的种群水平Bd估算数据，因此未将该物种纳入本研究的分析范畴。有关本研究中北部豹蛙的Bd感染详情，请参见补充文件S1中的表S3。 两栖动物群落及种群的Bd感染率统计。