Characterisation of the transcriptome of a wild great tit Parus major population by next generation sequencing. Parus major

RNA was extracted from eight tissues of wild great tit fledglings, and a normalised cDNA library generated and sequenced. Approximately 4.6 million sequences and 1.4 billion bases of DNA were generated and assembled into 95,979 contigs. Over 90% of contigs aligned with the zebra finch (Taeniopygia guttata) genome, and one third aligned with known Taeniopygia guttata (zebra finch) and Gallus gallus (chicken) transcripts. More than 35,000 single nucleotide polymorphisms and 10,000 microsatellite repeats were identified.本研究从野生大山雀雏鸟的8种组织中提取总RNA，构建归一化cDNA文库并开展测序。本次测序共生成约460万条序列、总计14亿碱基的DNA数据，并将其组装为95979个重叠群（contigs）。其中超过90%的重叠群可比对至斑胸草雀（Taeniopygia guttata）基因组，另有三分之一的重叠群可比对至已知的斑胸草雀与家鸡（Gallus gallus）转录本。本研究共鉴定得到超过35000个单核苷酸多态性（single nucleotide polymorphisms）以及10000个微卫星重复序列。