Gene expression profiling in 8 lung cancer in Xuanwei samples. Homo sapiens

In order to screen potential novel 'driver genes' in lung cancer in Xuanwei (LCXW), differentially expressed genes (DEGs) were detected on 8 paired LCXW and non-cancerous lung (NCL) tissues by gene expression microarrays. Statistically significantly expressed genes were identified using the mixed model analysis of variance with a false discovery rate (Benjamini-Hochberg test) adjusted p value of ≤ 0.05 and absolute fold-change values ≥ 2 or ≤ 0.5. A total of 5,129 genes were detected to be DEGs. Of these DEGs, 3,248 genes were upregulated, while another 1,881 genes were downregulated. Overall design: 8 paired LCXW and NCL tissues were tested.为筛选宣威肺癌（lung cancer in Xuanwei, LCXW）中潜在的新型驱动基因（driver genes），研究人员通过基因表达微阵列（gene expression microarrays）对8对宣威肺癌组织与配对的非癌性肺组织（non-cancerous lung tissues, NCL）进行差异表达基因（differentially expressed genes, DEGs）检测。采用混合模型方差分析，以错误发现率（false discovery rate, Benjamini-Hochberg检验）校正的P值≤0.05、绝对折叠变化（fold-change）值≥2或≤0.5为筛选标准，鉴定得到具有统计学显著性的差异表达基因。本研究共检出5129个差异表达基因，其中3248个为上调基因，另有1881个为下调基因。研究总体设计：共对8对配对的宣威肺癌组织与非癌性肺组织样本进行检测。