mRNA profiles of Flammulina velutipes which of anti-disease and early fruiting by RNA sequencing

We isolated a bacterial identified as the genus of Mycetocola from the infected Flammulina velutipes samples collected from mushroom house in Hebei, North China. The stipe of F. velutipes appeared water-soaked, soften and browning after infected, finally, the whole plant rotten. The incidence of the disease can reach more than 80% in the serious areas, resulting in an obvious decline in the yield and quality of the F. velutipes. Furthermore, the pathogenic bacteria may occurrence in large areas by accumulated in the annual cultivation process. Therefore, the most effective measures to control this bacterial disease is screening resistant strains of F. velutipes with high yield and good agronomic traits. We obtained a white F. velutipes strain JK502 which has strong resistance to this bacterium by using the double-layer plate method combined with the sporozoite hybridization. JK501, which can early fruited, was screened using the same parent SH9 by multi spore hybridization. In order to investigate the differences of gene expression between the parent strain and the selected strains, we completed the transcriptome sequencing by Illumina HiSeq X system. Flammulina velutipes mRNA profiles of the selected strains JK501, JK502 and the parent strain SH9 were generated by deep sequencing, in triplicate, by Illumina HiSeq X system.

本研究从中国华北河北省某菇房采集的感染金针菇（Flammulina velutipes）样本中，分离得到一株经鉴定属于Mycetocola属的细菌。染病金针菇的菌柄会出现水渍状软化、褐变症状，最终整株腐烂。该病害在重病区域的发病率可达80%以上，显著降低金针菇的产量与品质。此外，该病原菌可在年度栽培过程中逐年积累，进而引发大面积暴发。因此，防控该细菌性病害最有效的手段是筛选兼具高产与优良农艺性状的金针菇抗病菌株。本研究采用双层平板法（double-layer plate method）结合孢子囊杂交（sporozoite hybridization）技术，获得了一株对该病原菌具有强抗性的白色金针菇菌株JK502；同时以同一亲本SH9为材料，通过多孢子杂交（multi spore hybridization）技术筛选得到了早结菇菌株JK501。为探究亲本菌株与选育菌株间的基因表达差异，本研究采用Illumina HiSeq X测序系统（Illumina HiSeq X）完成转录组测序（transcriptome sequencing）。本研究通过Illumina HiSeq X测序系统对选育菌株JK501、JK502及亲本菌株SH9的金针菇mRNA进行深度测序，所有样本均设置3次生物学重复。