<i>Listeria</i> infection of melanoma induces nitric oxide and iNOS expression.

aB16F10 murine melanoma or BMDC were infected with LMWT (B16F10-LMWT, BMDC-LMWT) or non-infected (B16F10-NI, BMDC-NI) for 24 hours. bNO produced was measured in cell supernatants. Results are expressed as nmol of NO produced by 105 cells (mean ± SD, P<0.005) obtained with triplicate samples. cCell surfaces markers of B16F10 and BMDC infected or not with LMWT were analysed by FACS using the following antibodies: CD11c-PE, iNOS-FITC, CD86-V450 and MHC-II-APC. Samples were acquired using FACSCanto flow cytometer. Results are expressed as the percentages of positive cell (mean ± SD, P<0.005). Listeria infection of melanoma induces nitric oxide and iNOS expression.a. 将B16F10小鼠黑色素瘤细胞或骨髓源树突状细胞（BMDC）分别用LMWT感染24小时，设置感染组（B16F10-LMWT、BMDC-LMWT）与未感染对照组（B16F10-NI、BMDC-NI）。 b. 检测细胞上清液中生成的一氧化氮（NO）含量，结果以每10^5个细胞产生的NO纳摩尔数表示，数据为三次重复实验所得的平均值±标准差（mean ± SD, P<0.005）。 c. 采用流式细胞术（Fluorescence-Activated Cell Sorting, FACS）分析经LMWT感染或未感染的B16F10细胞与BMDC的细胞表面标志物，所用抗体包括CD11c-PE、iNOS-FITC、CD86-V450及MHC-II-APC；样本通过FACSCanto流式细胞仪完成数据采集。结果以阳性细胞百分比表示，数据为三次重复实验所得的平均值±标准差（mean ± SD, P<0.005）。 李斯特菌（Listeria）感染黑色素瘤可诱导一氧化氮（NO）及诱导型一氧化氮合酶（iNOS）的表达。