SH1045662.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE提供了一套统一的方法，用于界定、识别、交流以及开展基于DNA的物种假说（DNA-based Species Hypotheses，以下简称SH）相关工作。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，以下简称ITS）序列，均通过应用一组动态距离阈值（0.5%~3.0%）聚类至近似物种水平。所有SH均会被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，以下简称DOI）形式的名称，其分类学与生态学注释信息将通过分布式、依托网页的第三方注释流程完成核验。SH将结合该假说下所有序列的鉴定结果，尽可能与对应的分类单元名称及其分类层级（如门、纲、目等）建立关联。研究人员会选取一条自动或人工标定的序列作为每个SH的代表序列，此类代表序列已发布于https://unite.ut.ee/repository.php，可供科学界用于局部序列相似性检索、新一代测序分析流程等科研场景。随着公共真菌ITS序列库规模持续增长，该系统与数据集将自动完成更新。