Supplementary Figure S1 GSEA and ssGSEA analysis

Bubble plots exhibited the top-ranked GSEA results for the top-ranked KEGG pathways in (A) recurrent POP compared with primary POP uterosacral ligaments, and (B) recurrent POP compared with non-POP uterosacral ligaments. The classic GSEA plots showed that both adipose- and inflammation-related pathways were activated in the two contrast matrices for (C) recurrent POP vs.vs primary POP uterosacral ligaments, and (D) recurrent POP vs.vs non-POP uterosacral ligaments. Heatmaps showed hierarchical clustering of ssGSEA scores of KEGG pathways differentially enriched in (E) recurrent POP vs.vs primary POP uterosacral ligaments and (F) recurrent POP vs.vs non-POP uterosacral ligaments. (G) Spearman correlation analysis of pathway ssGSEA scores revealed that PPAR signaling pathways was strongly associated with adipose- and inflammation-related pathways in non-POP, primary POP and recurrent POP uterosacral ligaments. GSEA, g: Gene set enrichment analysis; ssGSEA, the s: Single-sample gene set enrichment analysis; POP, p: Pelvic organ prolapses; NES, n: Normalized enrichment scores.

气泡图呈现了两组对照中排名靠前的KEGG通路所对应的顶级基因集富集分析（Gene Set Enrichment Analysis，GSEA）结果：(A) 复发性盆腔器官脱垂（Pelvic Organ Prolapses，POP）与原发性POP骶子宫韧带组织的对照，以及(B) 复发性POP与非POP骶子宫韧带组织的对照。经典GSEA绘图结果显示，在(C)复发性POP与原发性POP骶子宫韧带、(D)复发性POP与非POP骶子宫韧带这两个对照矩阵中，脂肪相关通路与炎症相关通路均被激活。热图展示了(E)复发性POP与原发性POP骶子宫韧带、(F)复发性POP与非POP骶子宫韧带中差异富集的KEGG通路的单样本基因集富集分析（Single-sample Gene Set Enrichment Analysis，ssGSEA）得分的层级聚类结果。(G) 通路ssGSEA得分的斯皮尔曼相关性分析显示，在非POP、原发性POP及复发性POP的骶子宫韧带组织中，PPAR信号通路与脂肪相关通路、炎症相关通路呈显著相关性。术语说明：GSEA即基因集富集分析（Gene Set Enrichment Analysis）；ssGSEA即单样本基因集富集分析（Single-sample Gene Set Enrichment Analysis）；POP即盆腔器官脱垂（Pelvic Organ Prolapses）；NES即标准化富集得分（Normalized Enrichment Scores）。