Processed Plaque-2.0 infection scores of the PCL screen, imaged and analysed at EPFL.

PCL compounds are indicated (name). The Plaque2.0-based infection scores of the four biological replicates of each PCL compound were averaged (mean). The Plaque2.0-based infection scores of the positive and negative controls are averaged (mean) over the 32 technical replicates, each, per plate. Each compound’s scores were normalized by the mean score of the negative control of the respective plate and indicated by Mean N_nuclei (number of nuclei based on Hoechst staining), Mean N_infected (number of GFP reporter-based infected nuclei), Mean InfIndex (ratio of numberOfInfectedNuclei to numberOfNuclei), Mean N_plaques (number of GFP reporter-based plaques) and Mean TotVirInt (total GFP reporter signal intensity). Non-toxic compounds were filtered by applying an inclusive μ- (mean of the negative control)  2σ (SD of the negative control) threshold for number of nuclei. Efficacy was filtered by applying an excluding μ-  3σ (SD of the negative control) threshold for the infection scores. The obtained scores for each infection score of each PCL compound indicated as Mean Scores N_nuclei (number of nuclei based on Hoechst staining), Scores N_Infected (number of GFP reporter-based number of infected nuclei), Scores InfIndex (ratio of numberOfInfectedNuclei to numberOfNuclei), Scores N_plaques (number of GFP reporter-based plaques) and Scores TotVirInt (total GFP reporter signal intensity). Compounds exhibiting significant toxicity to noninfected cells were excluded.本研究中所有PCL化合物均已标注其名称。针对每种PCL化合物的4次生物学重复，其基于Plaque2.0方法得到的感染评分将取算术平均值。每块检测板的阳性对照与阴性对照的Plaque2.0感染评分，均分别基于该板内的32次技术重复取算术平均值。 每种化合物的评分将以对应检测板的阴性对照平均评分进行标准化，并通过以下指标表征：平均细胞核数（Mean N_nuclei，基于Hoechst染色的细胞核计数）、平均感染细胞核数（Mean N_infected，基于绿色荧光蛋白（GFP）报告基因标记的感染细胞核计数）、平均感染指数（Mean InfIndex，感染细胞核数与总细胞核数的比值）、平均噬斑数（Mean N_plaques，基于GFP报告基因标记的噬斑计数）以及平均总GFP报告基因信号强度（Mean TotVirInt，GFP报告基因总信号强度）。 通过针对细胞核数量设置阴性对照均值（μ）±2倍标准差（σ）的包容性阈值，筛选出无细胞毒性的化合物。针对感染评分设置阴性对照均值（μ）±3倍标准差（σ）的排他性阈值，完成活性筛选。 每种PCL化合物的各项感染评分最终将以如下评分指标呈现：细胞核数评分（Scores N_nuclei，基于Hoechst染色的细胞核计数）、感染细胞核数评分（Scores N_Infected，基于GFP报告基因标记的感染细胞核计数）、感染指数评分（Scores InfIndex，感染细胞核数与总细胞核数的比值）、噬斑数评分（Scores N_plaques，基于GFP报告基因标记的噬斑计数）以及总GFP报告基因信号强度评分（Scores TotVirInt，GFP报告基因总信号强度）。对未感染细胞具有显著细胞毒性的化合物将被排除。