mRNA analysis in different mouse tissues. Mus musculus

The functioning of a specific tissue depends on the expression pattern of the different genes. We used microarrays to compare gene expression across different murine tissues, to get a better understanding in the expression pattern and functioning of the different tissues. With this analysis, we were not only able to identify genes that were specifically expressed in a spicific tissue but, as important, we also identified genes that were specifically repressed in a tissue, compared to al the other analysed tissues. Overall design: C57Bl6/j6 (Janvier) mice between 10 and 12 weeks old were used to isolate 22 different tissues. Mainly male mice were used, except for placenta and ovary, where female mice were used. Also the mouse insulinoma cellline MIN6 was analysed in this study. For every tissue, at least 3 biological replicates were used.

特定组织的功能依赖于不同基因的表达模式。我们利用微阵列（microarrays）对不同小鼠组织的基因表达谱进行比较分析，以更深入地理解各类组织的表达模式与功能机制。通过本次分析，我们不仅成功鉴定出在特定组织中特异性表达的基因，同等重要的是，我们还识别出相较于其他所有分析过的组织，在某一组织中特异性受抑制的基因。
总体实验设计：本研究选用10至12周龄的C57Bl6/j6（Janvier）小鼠，从中分离得到22种不同组织。除胎盘和卵巢使用雌性小鼠外，其余组织主要以雄性小鼠作为实验材料。本研究同时对小鼠胰岛素瘤细胞系MIN6进行了分析。所有组织样本均至少设置3次生物学重复。