S4B Figure

S4B Fig. IFN-β induction during KSHV lytic reactivation requires all three IFN-β enhanceosome transcription factors. BC-3 cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and where indicated, 1 μM of the dual AP-1 and NF-κB inhibitor SP100030. A. RNA was extracted and IFN-β mRNA levels were measured by RT-qPCR, normalized to 18S rRNA, and plotted relative to the sample treated with TPA, casp-i, and vehicle control (“(-)”). Cells were stained for KSHV ORF45 and analyzed by flow cytometry. n= 5.