S4B Figure

S4B Fig. IFN-β induction during KSHV lytic reactivation requires all three IFN-β enhanceosome transcription factors. BC-3 cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and where indicated, 1 μM of the dual AP-1 and NF-κB inhibitor SP100030. A. RNA was extracted and IFN-β mRNA levels were measured by RT-qPCR, normalized to 18S rRNA, and plotted relative to the sample treated with TPA, casp-i, and vehicle control (“(-)”). Cells were stained for KSHV ORF45 and analyzed by flow cytometry. n= 5.S4B 图。卡波西肉瘤相关疱疹病毒（KSHV）裂解再激活过程中的干扰素-β（IFN-β）诱导需要全部三种干扰素-β增强子复合体转录因子。将BC-3细胞以20 ng/ml的佛波酯（TPA）处理48小时以诱导裂解周期（记为"lytic (+TPA)"），同时添加10 μM的泛半胱天冬酶抑制剂IDN-6556（记为"casp-i"）；若实验有标注，还会加入1 μM的AP-1与NF-κB双靶点抑制剂SP100030。子图A：提取细胞总RNA，通过逆转录实时定量聚合酶链反应（RT-qPCR）检测IFN-β mRNA水平，以18S核糖体RNA（18S rRNA）作为内参进行归一化，并以仅接受TPA、casp-i与溶剂对照处理的样品（记为"(-)"）为参照绘制相对表达量；随后对细胞进行KSHV ORF45（KSHV ORF45）染色，采用流式细胞术进行分析。本实验重复次数n=5。