Phylogenetically under‐dispersed gut microbiomes are not correlated with host genomic heterozygosity in a genetically diverse reptile community

We are providing semi-processed datasets relevant to the paper \"Phylogenetically under-dispersed gut microbiomes across a range of host genetic diversity in a reptile community point to structuring by conserved host genes.\" Specifically, we include VCF files of RADseq data from host individuals, which are processed versions of the raw reads available at NCBI's Short Read Archive under PRJA744273. These data were processed for heterozygosity calculation using an adapted of the pipeline presented in Singhal et al. 2017, \"Genetic diversity is largely unpredictable but scales with museum occurrences in a species-rich clade of Australian lizards.\"
In addition, we include a database of 16S sequences from gut microbiome amplicon sequencing from the same host animals. The raw reads are available at NCBI's Short Read Archive under PRJNA746253. The sequences accessioned here are a curated, cleaned set of reference reads to which we realigned reads from each individual host.

本数据集关联于论文"《爬行动物类群中不同宿主遗传多样性水平下的系统发育欠分散肠道微生物组表明其群落结构由保守宿主基因塑造》"，现予以公开。具体而言，本数据集包含宿主个体的限制性位点相关DNA测序（Restriction-site Associated DNA sequencing，RADseq）数据的变异识别格式（Variant Call Format，VCF）文件，该类文件为NCBI短读长存档库（Short Read Archive，SRA）中PRJA744273号存档下原始读段的处理后版本。本数据集的RADseq数据采用改编自Singhal等人2017年发表的研究流程进行处理，以用于杂合度计算，该原始研究题为"《澳大利亚蜥蜴物种丰富演化支的遗传多样性大多难以预测，但与博物馆馆藏记录规模正相关》"。此外，本数据集还包含来自同一批宿主动物的肠道微生物组扩增子测序的16S序列数据库，其原始读段可在NCBI短读长存档库中通过PRJNA746253号存档获取。本次存档的序列为经过人工整理与质控的参考读段集，我们已将所有宿主个体的测序读段重新比对至该参考序列集上。