RNA-seq of primary tumor-derived human gastric cancer stem cells (GCSCs) and differentiated cells (GCSCs treated with serum)

We performed RNA-seq analysis of human gastric cancer stem cell treated with or without serum to analyze the differentially expressed genes between GCSCs and differentiated cells, and find the potential key factors in GCSCs. Fresh isolated, primary tumor-derived GCSC cells were cultured with or without serum for 6 days, and then were collected for TRIzol lysed for RNA extraction. 6 RNAseq samples across 2 conditions:SP (sphere, without serum) (n=3) and AD (adhering, with serum) (n=3)

本研究针对经血清处理与未经血清处理的人胃癌干细胞（gastric cancer stem cell, GCSCs）开展RNA测序（RNA-seq）分析，旨在对比GCSCs与分化细胞间的差异表达基因，并挖掘GCSCs中潜在的关键调控因子。实验将新鲜分离获得的原代肿瘤来源GCSCs分别在含血清与不含血清的培养基中培养6天，随后收集细胞并使用TRIzol试剂裂解以提取RNA。本数据集共包含6个RNA测序样本，分为2组培养条件：SP组（悬浮培养，无血清，n=3）与AD组（贴壁培养，含血清，n=3）。