Identification of NOTCH3 unique target genes

Notch signaling is an evolutionarily conserved signaling pathway.NOTCH3 signaling has been shown to potentially paly an important role in luminal cell fate commitment in human breast tissue by regulating unique target genes We overexpressed the intracellular domain of NOTCH1 (N1ICD) and NOTCH3 (N3ICD) receptor in nonmalignant human breast cell line, 184-hTerts 184-hTert cells were lentivirally transduced with either empty vector control, N1ICD or N3ICD. RNA was isolated for affymetrix microarray analysisNotch信号通路（Notch signaling）是一类进化保守的信号通路。已有研究表明，NOTCH3信号通路（NOTCH3 signaling）可通过调控独特的靶基因，在人类乳腺组织的腔细胞命运决定中发挥潜在重要作用。本研究在非恶性人类乳腺细胞系184-hTerts与184-hTert细胞中，过表达NOTCH1的细胞内结构域（N1ICD）及NOTCH3的细胞内结构域（N3ICD）；具体操作为：以空载体作为对照，通过慢病毒转导分别将空载体、N1ICD或N3ICD导入上述细胞。后续提取细胞总RNA，用于Affymetrix基因芯片分析（Affymetrix microarray analysis）。