Characterization of macrophages influence in human melanoma cells

Tumor cells attract and dynamically interact with monocytes/macrophages, one of the more abundant cell types in the tumor microenvironment, to subvert their differentiation into tumor associated macrophages (TAMs), which mainly promote immunosuppression and neoplastic progression, but the mechanisms governing their protumoral activity are not completely understood. Thus, identifying molecular pathways promoted by macrophages that could be responsible for immune evasion and tumor malignicity may provide new therapeutic targets to improve the efficacy of immunotherapy of cancer. BLM human melanoma cell line was coculture for 24h with in vitro differentiated macrophages, obtained from CD14+ monocytes isolated from human PBMCs in the presence of GM-CSF or M-CSF for seven days. All samples have been processed in triplicates.肿瘤细胞可招募并与单核细胞/巨噬细胞动态互作——后者是肿瘤微环境中丰度较高的细胞类群之一——以劫持其分化方向，使其转化为肿瘤相关巨噬细胞（tumor associated macrophages, TAMs）。这类细胞主要介导免疫抑制并促进肿瘤发生发展，但调控其促肿瘤活性的分子机制尚未完全阐明。 因此，探究巨噬细胞中介导免疫逃逸与肿瘤恶性进展的调控分子通路，可为提升癌症免疫治疗疗效提供全新的治疗靶点。 本数据集采用BLM人黑色素瘤细胞系，与经体外分化获得的巨噬细胞共培养24小时；该巨噬细胞由从人外周血单个核细胞（peripheral blood mononuclear cell, PBMC）中分离的CD14+单核细胞，在粒细胞-巨噬细胞集落刺激因子（GM-CSF）或巨噬细胞集落刺激因子（M-CSF）存在的条件下诱导分化7天所得。所有样本均设置三次平行重复实验。